fastprotein bluematrix (Valiant Co Ltd)

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Valiant Co Ltd fastprotein bluematrix
Fastprotein Bluematrix, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fastprotein+blue/FastProtein+Blue+Matrix/pm32281733-45-18-20
Average 90 stars, based on 41 article reviews

fastprotein bluematrix - by Bioz Stars, 2026-09

90/100 stars

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Isolation:

Article Title: Repression of Staphylococcus aureus SrrAB using inducible antisense srrA alters growth and virulence factor transcript levels.
Article Snippet: Our laboratory has previously identified the staphylococcal respiratory response (SrrAB), a Staphylococcus aureus two-component system that acts in the global regulation of virulence factors.. In strain RN4220, SrrAB downregulated production of agr RNAIII, protein A, and TSST-1, particularly under low-oxygen conditions.. Work by another group showed that SrrAB regulates energy metabolism genes and indicated that SrrAB may regulate energy transduction in response to changes in oxygen availability.

Purification:

Incubation:

Article Title: Mechanism of plasmid-mediated quinolone resistance
Article Snippet: .. Aliquots were added to 3 FastProtein Blue (Qbiogene) tubes, vortexed for 5 min, and centrifuged for 1 min at 10,000 × g . The supernatant was collected and incubated in a 1.5-ml tube with Ni-NTA resin at a 4:1 ratio by volume on a rocking platform for 2 to 3 h to allow the His 6 -tagged protein to bind to the resin. ..

DNA Extraction:

Article Title: Immunohistochemical and Taqman real-time PCR detection of mycobacterial infections in fish.
Article Snippet: Real-time PCR and immunohistochemistry (IHC) assays were developed to detect fish mycobacterial infections at the genus level, based on the RNA polymerase b subunit (rpoB) gene and polyclonal anti-Mycobacterium rabbit serum, respectively.. The PCR assay positively identified a number of pathogenic mycobacteria including Mycobacterium abscessus, M. avium ssp. avium, M. bohemicum, M. chelonae ssp. chelonae, M. farcinogenes, M. flavescens, M. fortuitum ssp. fortuitum, M. gastri, M. gordonae, M. immunogenicum, M. malmoense, M. marinum, M. montefiorense, M. phlei, M. phocaicum, M. pseudoshottsii, M. salmoniphilum, M. senegalense, M. shottsii, M. smegmatis, M. szulgi and M. wolinskyi.. A detection limit equivalent to 10 cfu g was registered for M. salmoniphiluminfected fish tissue.

Disruption:

Bacteria:

Article Title: Generation of Branched-Chain Fatty Acids through Lipoate-Dependent Metabolism Facilitates Intracellular Growth of Listeria monocytogenes
Article Snippet: Bacteria were prepared and analyzed by immunoblotting with an anti-lipoic acid antibody (Calbiochem) as previously described ( 31 ), except that washed exponential-phase cultures (at an optical density at 600 nanometers [OD 600 ] of 0.5) or stationary-phase cultures (OD 600 of 1.2) were used after growth in BHI medium at 37°C. .. Bacteria were pelleted and then lysed with FastProtein Blue lysing matrix (MP Biomedicals) by processing in a FastPrep machine for 40 s at an intensity setting of 6.0. .. Lactate was quantified using a lactate assay kit (BioVision; catalog no. K607-100), which was quantified by a colorimetric change at 570 nm.

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